Coding

Part:BBa_K5250006:Design

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-09-30)


DGC PisoF_00565 + Tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 424
    Illegal PstI site found at 493
    Illegal PstI site found at 711
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 424
    Illegal PstI site found at 493
    Illegal PstI site found at 711
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 424
    Illegal PstI site found at 493
    Illegal PstI site found at 711
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 424
    Illegal PstI site found at 493
    Illegal PstI site found at 711
    Illegal NgoMIV site found at 732
    Illegal NgoMIV site found at 855
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for pseudomonas species IsoF. It contains a His-tag for protein purification.


Source

The genetic sequence of this part was obtained from a Pseudomonas species IsoF gene bank provided to us by our host lab.

References